Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional cultures

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Investigating Conversion of Endplate Chondrocytes Induced by Intermittent Cyclic Mechanical Unconfined Compression in Three-Dimensional Cultures

Mechanical stimulation is known to regulate the calcification of endplate chondrocytes. The ANK protein has a strong influence on anti-calcification by transports intracellular inorganic pyrophosphate (PPi) to the extracellular. It is known that TGF-β1 is able to induced Ank gene expression and protect chondrocyte calcification. Intermittent cyclic mechanical tension (ICMT) could induce calcifi...

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Costal Versus Articular Chondrocytes in Alginate Three-Dimensional Cultures

Given the difficulties in accessing articular cartilage as a source of chondrocytes to be used in fabricating cartilage constructs, alternative sources are required. The present study examined chondrocytes from costal cartilage for their suitability in cartilage tissue engineering. Chondrocytes isolated from rat knee and rib hyaline cartilage were separately mixed with alginate and placed in a ...

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Intermittent cyclic mechanical tension altered the microRNA expression profile of human cartilage endplate chondrocytes

Previous studies have identified the association between cartilage endplate (CEP) degeneration and abnormal mechanical loading. Several studies have reported that intermittent cyclic mechanical tension (ICMT) regulates CEP degeneration via various biological processes and signaling pathways. However, the functions of microRNAs in regulating the cellular responses of CEP chondrocytes to ICMT rem...

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Unconfined creep compression of chondrocytes.

The study of single cell mechanics offers a valuable tool for understanding cellular milieus. Specific knowledge of chondrocyte biomechanics could lead to elucidation of disease etiologies and the biomechanical factors most critical to stimulating regenerative processes in articular cartilage. Recent studies in our laboratory have suggested that it may be acceptable to approximate the shape of ...

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costal versus articular chondrocytes in alginate three-dimensional cultures

given the difficulties in accessing articular cartilage as a source of chondrocytes to be used in fabricating cartilage constructs, alternative sources are required. the present study examined chondrocytes from costal cartilage for their suitability in cartilage tissue engineering. chondrocytes isolated from rat knee and rib hyaline cartilage were separately mixed with alginate and placed in a ...

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ژورنال

عنوان ژورنال: European Journal of Histochemistry

سال: 2014

ISSN: 2038-8306,1121-760X

DOI: 10.4081/ejh.2014.2415